![]() HBV and HCV infection is usually diagnosed by the detection of anti-HBV/or anti-HCV antibodies in a patient's serum that react to recombinant HBV or HCV proteins in an enzyme immunoassay or chemiluminescence immunoassay. An estimated 30% of people infected develop liver cirrhosis and/or hepatocellular carcinoma (HCC). According to the World Health Organization (WHO), 130–150 million people are infected with HCV worldwide and 240 million people are chronically infected with HBV. The real-time quantitative PCR assay developed in this study provides an ideal system for routine diagnosis and confirmation of indeterminate serological results, especially in immunosuppressed patients.Īcute and chronic infections with hepatitis B virus (HBV) or hepatitis C virus (HCV) lead to significant mortality and are a major public health problem worldwide. In sera from patients infected with hepatitis B virus or hepatitis C virus viral loads (19 IU/mL and 1.9 × 10 9 IU/mL), we quantified viral loads with a detection limit of 1.9 × 10 2 IU/mL. The standard curve showed a linear relationship from 19 IU/mL to 1.9 × 10 9 IU/mL of serum, with a coefficient of determination ( r 2) of 0.99. ![]() A correlation coefficient of 0.983 and 0.963 for hepatitis B virus and hepatitis C virus, respectively, was obtained based on cycle threshold values and concentrations of DNA or RNA. For standardization and validation of the assay, an international panel of hepatitis B virus/hepatitis C virus and standard plasmids was used. ![]() In this study, we developed an assay using specific primers and probes to quantify hepatitis B virus DNA or hepatitis C virus RNA in serum from infected patients. The quantification of viral nucleic acids in serum by real-time PCR plays an important role in diagnosing hepatitis B virus and hepatitis C virus infection. ![]()
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